• Product NameUCP3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total UCP3 protein.
• Immunogen DescriptionSynthetic peptide of human UCP3
• Target NameUCP3
• Alternative Names    SLC25A9
• Accession No. Swiss-Prot#: P55916
  Gene ID: 7352
• UniprotP55916
• Gene ID7352;
• Calculated MW 34kd
• Concentration 1.2mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:20-1:100

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane.

• Gel: 8%SDS-PAGE
  Lysate: 40 μg
  Lane: Mouse lung tissue
  Primary antibody: 1/200 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 1 minute
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #43430 at dilution 1/20.
• Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using #43430 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting